MAGGIE will provide robust GTL technologies and comprehensive characterization to efficiently couple gene sequence and genomic analyses with protein interactions and thereby elucidate functional relationships and pathways. The operational principle guiding MAGGIE objectives can be succinctly stated: protein functional relationships involve interaction mosaics that self-assemble from independent protein pieces that are tuned by modifications and metabolites. MAGGIE builds strong synergies among the Components to address long term and immediate GTL objectives by combining the advantages of specific microbial systems with those of advanced technologies. The objective for the proposed 5-year MAGGIE Program is therefore to comprehensively characterize the Protein Complexes (PCs) and Modified Proteins (MPs) underlying microbial cell biology. A compelling overall goal is to help reduce the immense complexity of protein interactions to interpretable patterns though an interplay among experimental efforts of MAGGIE Program members in molecular biology, biochemistry, biophysics, mathemathics, computational science, and informatics. MAGGIE will address immediate GTL missions by accomplishing three specific goals:

1. provide a comprehensive, hierarchical map of prototypical microbial PCs and MPs by combining native biomass and tagged protein characterizations from hyperthermophiles (temperature-trapping otherwise reversible protein interactions) with comprehensive systems biology characterizations of a non-thermophilic model organism,
2. develop and apply advanced mass spectroscopy and SAXS technologies for high-throughput characterizations of PCs and MPs,
3. create and test powerful computational descriptions for protein functional interactions.

Current MAGGIE GTL News