MAGGIE GTL Component 5:

Small-Angle X-ray Scattering (SAXS)

Component Director:

Component Goals:

1. To develop facilities, coordination and conduct high throughput SAXS data collection on PC and MP samples provided by MAGGIE components 1, 2, and 3. High throughput sample format and buffer conditions will be closely coordinated with relevant components. PC structural parameters will be monitored both in response to the addition of small ligands and as a function of temperature.


2. To automate and accelerate SAXS data processing and quality validation for protein shape, conformational changes, assembly state and temperature response and provide feedback to MAGGIE components 1, 2 and 3 on stabilizing conditions for both SAXS and other techniques. Effect of conditionally induced conformation changes induced by small ligands on PC structural parameters will be characterized and added to prepared databases.


3. To validate a small subset of results from shape restoration from SAXS analysis using existing crystallography capabilities of the SIBYLS beamline.


4. To enhance SAXS technologies and data production by identifying cryo-cooling conditions and protective additives to reduce radiation damage. In addition, we will explore the use of synchrotron footprinting in combination with the Component 4 mass spectrometry to map interfaces.


5. To construct and maintain a database of results from SAXS cataloging mass, Rg, Dmax, and shape reconstruction for cross-referencing both within the MAGGIE (ADM Core, Component 6) and other databases.