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In general, 10-100 µL of 1mg/ml sample is more than enough sample for analysis. If the sample is a solid, 100ug would be sufficient.
All instruments are capable of detecting down to the 1 µg/mL level. However, it is good practice to submit around 1 mg/mL. In the event that the concentration is unknown, enter 1mg/mL in the concentration box and in the “Comments and special instructions” box, please state “concentration unknown.”
Submitting a solid is also possible, please provide the amount submitted along with the appropriate solvent in which to dissolve the solid so we can determine how much of which solvent to use.
The solvents most preferred are methanol and water. Chloroform and dichloromethane are also acceptable. Avoid those solvents with high boiling points (such as DMSO) as they are particularly mass spec unfriendly. For a list of salt/buffer tolerances for each method of MS, please see salt tolerance table.
All the instruments are quite sensitive and concentration of 10-100µM usually give a good response. Very high concentration sample can saturate the detector, and hinder the ionization or cause carryover to other samples. MeOH is the typical dilution solvent (DCM for GCMS), if methanol is an unacceptable diluent, please specify an appropriate substitute in the “Comments and special instructions” box of the sample submission form.
If absolutely necessary, you may submit in DMSO but most likely, your sample will need to be diluted into a mass-spec-friendly solvent like methanol.
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